Notice: Never centrifuge the Delivery Cartridge at this stage as this will likely produce loss in shipping and delivery effectiveness. Carefully tap the Delivery Cartridge numerous moments to gather quantity at The underside if necessary.
As soon as the run is total, retrieve the Shipping and delivery Cartridge through the instrument. The cell sample really should be at The underside or facet of the gathering tube. Note: It is usually recommended to spin down the Shipping Cartridge in a very mini-centrifuge for your couple seconds for comprehensive quantity Restoration.
Move cytometry can be utilized to evaluate the effectiveness of differentiation protocols With all the STEMdiff™ system, by checking out specific marker expression at Just about every stage with the differentiation procedure. It can even be used for other programs, which include cell sorting, immunophenotyping, and purity assessment.
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Notice: For an exceptionally confluent culture, cultures may be a bit additional clumpy all through dissociation. To beat this, Carefully scrape the monolayer using a pipette suggestion just after adding the dissociation reagent to facilitate the dissociation during incubation.
You may find out strategies to receive great-high-quality neural progenitor cells, which might be vital for downstream differentiation, as well as several cryopreservation points to save lots of time and enhance regularity. She also talks with regards to the protein and transcriptional profiles and autophagy levels of astrocytes created utilizing serum-no cost media.
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